Karpagam Arts And Science College

DEPARTMENT OF BIOCHEMISTRY

ABOUT US
Department of Biochemistry offers both B.Sc. and M.Sc. courses and M.Phil. and Ph.D. Research programmes. The department includes eight faculty members of which, 3 are Ph.D holders, 4 are M.Phil holders and 1 PG holder. The Teachers pursue courses for research degrees. The Department has conducted Seminars and Conferences at National and State levels. As a part of extension activity the Department also has a Blood Donors Club in which we have donated about 500 units of blood and every year we have conducted blood group screening camp and diabetic screening camp in adopted villages. Also the work has been appreciated by Tamil Nadu State AIDS Control & State Blood Transfusion Soceity.
FACULTY
Name	Qualification	Designation
DR.V.K.GOPALAKRISHNAN	MSC,PHD,PDF(USA)	PROFESSOR
UMA.C	B.Ed,M.Phil.,(Ph.D)	READER
DEVAKI.K	M.SC,M.Phil.,(Ph.D)	READER
R.SHARMILA DEVI	MSC,M.PHIL	LECTURER
V.SUSEELA	M.Sc.,M.Phil	LECTURER
K.POORNIMA	M.Sc., B.ED., M.PHIL	LECTURER
SUNITHA.M	M.Sc.,M.Phil., (Ph.D.)	LECTURER
KALYANI	M.Sc,,M.Phil	LECTURER
P.SATHIYAPRIYA	M.Sc., (Ph.D)	LECTURER
JAYARAJ.N	M.SC., PGDCA	LECTURER
NIRMALA.C	M.SC.	LECTURER

PROJECTS
* Completed
	Improvement of infrastructure facilities in the existing Botanical garden for the conservation of rare and endemic species.
	Funding agency	: Ministry of Environment & Forests, New Delhi .
	Grant	: Rs. 2 Lakhs.
	In vitro screening and phytochemical characterization of selected Indian medicinal plants for their antimicrobial activity.
	Funding agency	: University Grants Commission (UGC), New Delhi .
	Grant	: Rs. 50,000/-

* Completed
	Treatment of textile effluents by Parthenium activated carbon adsorption and Bioremediation
	Funding agency	: University Grants Commission (UGC), New Delhi .
	Grant	Rs.10,08,100/-


	Research Projects (Ongoing) 1)University Grants Commission (UGC) Funded Major ResearchProject on “ Expression of transfected FHIT gene in ovarian cancer cell lines” Amount sanctioned for the period of three years(01-4-2007-31-03-2010) Rs-8, 03,600.00 2) Indian Council of Medical and Research (ICMR) Funded Major Research Project on “ Anticancer effect of Medicinal Plants on Prostate Cancer Progression Model in vitro” Amount sanctioned for the period of one year (15-05-2007 – 14-5-2008) Rs.4, 44,931.00 Research Prostate Cancer & Ovarian Cancer The mechanism of anticancer effect of medicinal plants against prostate cancer cell lines (PC-3, DU-145 and LNCaP) is done. The following medicinal plants are used for our study. 1. Ammania baccifera 2. Citrullus colocynthis Linn. 3. Lycopersicon esculentum 4. Crataeva nurvala 5. Tribulus terrestris FHIT is a candidate tumor suppressor gene. We are interested in finding out the expression of tranfected FHIT on ovarian cancer cell lines (SW626 and OVCAR-3). The elucidation of gene function and complex mechanisms of gene regulation in an organism is among the most challenging tasks for modern molecular biology and medicine.

	SEMINARS
	National seminar On ENVIRONMENTAL POLLUTION, Inaugurated by Dr.K.Subramanian Director, Institute of Forest Genetics & Tree Breeding, Coimbatore on 12TH DECEMBER 1997 National seminar On ‘EMERGING TRENDS IN ENERGY AND ENVIRONMENT’ Inaugurated by Dr.A Abdul Rahman, Emeritus Scientist, on 27TH JULY 2001 National seminar On BROAD SPECTRUM OF ENZYMES – THE EMERGING TECHNOLOGY, was inaugurated by Dr.A.Ashok Pandey, Scientist, Regional Research Laboratory, Trivandrum. National seminar On ‘FRONTIERS IN ENZYME TECHNOLOGY was Inaugurated by Dr.C.B.Vig, Luminous Biotech, K- Genic group, on Mumbai 15-16TH NOV 2002. National Level Symposium on Drug Discovery (A novel Approach) was inaugurated by Dr. Udhaya Bhaskara Rao on 17.09.04. National level Conference on “Technological Advancement in Clinical Biochemistry”, Inaugurated by Dr. R. Saraswathy M.S. Dean, Coimbatore medical college & hospital Coimbatore on 15.10.2004. National level Seminar on “Challenges for Green Environment”, 22.02.2006. was Inaugurated by Dr. Sabu Thomas scientist Rajiv Gandhi Center for Biotechnology Trivandrum, Kerala National level Seminar on “CHALLENGING DIABETES”, Karpagam Arts and Science College, 20.09.2006. inaugurated by Dr. Sreejith N. Kumar Chairman Indian Diabetes Education Association(IDEA) Trivandrum, Kerala. State level symposium on “MANAGEMENT OF DIABETES” inaugurated by Dr. Giri M.D PGDipDC Director-Regional Diabetic center , K.G Hospitals, Coimbatore on 03.02.07 National level Seminar on “BIODIVERSITY AND CONSERVATION”, inaugurated byDr.N.C Anil Kumar, Sr.Scientist, State Remote sensing Centre, Trivandrum, Kerala. on 16.02.2007. National level Seminar on “ONCOLOGICAL SURVEILLANCE ”, inaugurated by Dr.N.Srinivasan Director, University of Madras, Tharamani Campus, 31.08.2007 There is a proposal to conduct an international seminar on Nano technology and Cancer biology by the month of August 2008

BOTANICAL GARDEN

A Botanical garden was created by our Department in 2 acres of land within the campus. The existing Botanical garden has been upgraded with a funding of 2 lakhs from the Ministry of Environment and Forests, New Delhi for propagation of rare and endemic plant species.

LIST OF MEDICINAL PLANTS IN BOTANICAL GARDEN

1. Justicia beddomei (C.B.Clarke) Bennet (= Adhatoda beddomei C.B.Clarke)	ACANTHACEAE
2. Acalypha fruticosa Forssk	EUPHORBIACEAE
3. Aegle marmelos (L.) Correa	RUTACEAE
4. Alpinia calcarata Roscoe	ZINGIBERACEAE
5. Andrographis paniculata (Burm.f.) Wall. ex Nees	ACANTHACEAE
6. Aristolochia tagala Cham.	ARISTOLOCHIACEAE
7. Aristolochia indica L.	ARISTOLOCHIACEAE
8. Asperagus racemosus Willd.	LILIACEAE
9. Bauhinia racemosa Lam.	CAESALPINIACEAE
10.Canarium strictum Roxb.	BURSERACEAE
11. Cardiospermum canesens Wall.	SAPINDACEAE
12. Caralluma adscendens (Roxb.) Haw var. attenuata (Wight) Grave and Mayur.	ASCLEPIADACEAE
13. Cassia alata L.	CAESALPINIACEAE
14. Centella asiatica (L.) Urban	APIACEAE
15.Cinnamomum macrocarpum Hook.f.	LAURACEAE
16.Cissus quadrangularis L.	VITACEAE
17.Coccinia grandis (L.) Voigt	CUCURBITACEAE
18.Coscinium fenestratum (Gaertn.) Coleb.	MINISPERMACEAE
19.Curculigo orchioides Gaertn.	HYPOXIDACEAE
20.Cycas circinalis L.	CYCADACEAE
21.Cyclea peltata ( Lam.) Hook.f.	MINISPERMACEAE
22.Diospyros malabarica (Desr.) Kostel.	EBENACEAE
23.Garcinia gummi – gutta (L.) Robs.	CLUSIACEAE
24.Garcinia morella (Gaertn.) Desr.	CLUSIACEAE
25.Grewia tiliaefolia Vahl	TILIACEAE
26.Gymnema montanum (Roxb.)Hook.f.	ASCLEPIADACEAE
27.Gymnema sylvestre (Retz.) R.Br.ex Schultes	ASCLEPIADACEAE
28.Hedychium coronarium Koen.	ZINGIBERACEAE
29.Janakia arayalpathra Joseph & Chandras.	PERIPLOCACEAE
30.Jatropha curcas L.	EUPHORBIACEAE
31.Kalanchoe pinnata (Lam.) Pers.	CRASSULACEAE
32.Leea indica (Burm.f.) Merr.	LEEACEAE
33.Helictres isora L.	STERCULIACEAE
34.Hiptage benghalensis (L.) Kurz	MALPIGHIACEAE
35.Holarrhena antidysentrica (Roxb.ex Fleming) Wallich	APOCYNACEAE
36.Limonia acidissima L.	RUTACEAE
37.Madhuca longifolia (Koen.) Macbr.	SAPOTACEAE
38.Melia dubia Cav.	MELIACEAE
39.Mucuna atropurpurea DC.	FABACEAE
40.Myristica fragranss Houtt.	MYRISTICACEAE
41.Phyllanthus emblica L.	EUPHORBIACEAE
42.Plectranthus vettiveroides (Jacob) Singh & Sharma	LAMIACEAE
43.Pongamia pinnata (L.) Pierre	FABACEAE
44.Pterocarpus santalinus L.f.	FABACEAE
45.Rauvolfia serpentina (L.) Benth. ex Kurz	APOCYNACEAE
46.Rhinacanthus nasutus (L.) Kurz	ACANTHACEAE
47.Santalum album L.	SANTALACEAE
48.Saraca asoca (Roxb.) Wilde	CAESALPINIACEAE
49.Spilanthes calva DC.	ASTERACEAE
50.Strychnos potatorum L.f.	LOGANIACEAE
51. Justicia beddomei (C.B.Clarke) Bennet (= Adhatoda beddomei C.B. Clarke)	ACANTHACEAE
52 Rhinocanthus nasutus (L.)Kurz var. nasutus	ACANTHACEAE
53. Andrographis paniculuta (Burm. f.) Wall. ex Nees	ACANTHACEAE
54 . Scaevola sericea Vahl (= S.frutescens K. Krause)	GOODENIACEAE
55. Spilanthes calva DC. (= S. acmella auct.non (L.) Murr.)	ASTERACEAE
56. Kalanchoe sp.	CRASSULACEAE
57. Centella asiatica (L.) Urban	APIACEAE
58. Alpinia calcarata Roscoe	ZINGIBERACEAE
59. Trichopus zeylanicus Gaertn. Subsp. travancoricus (Beddome) Burkill	DIOSCOREACEAE
60. Cissus quadrangularis L.	VITACEAE
61. Mimosa pudica L.	MIMOSACEAE
62.Saraca asoca (Roxb.) Wilde	CAESALPINIACEAE
63.Filicium decipiens (Wight & Arn.)Thwaites	SAPINTACEAE
64.Strychnos potatorum L.f.	LOGANIACEAE
65.Antiaris toxicaria (Pers.) Lesch.	MORACEAE
66.Phyllanthes sp.	EUPHORABIACEAE
67.Euphorbia susan – holmesiae Binojk. & Gopalan	EUPHORBIACEAE
68.Wedelia chinensis (Osbeck) Merr.	ASTERACEAE.
69. Sauropus androgynous ((Multivitamin plant chekurmani)	EUPHORBICEAE
70. Elaphoglossum stigmatolepis	ELAPHOGLOSSACEAE
71. Elaphoglossum beddomei	ELAPHOGLOSSACEAE
72. Sphaeropteris crinita	CYANTHEACEAE
73. Diplazium travancoricum	ATHYRIACEAE
74. Amomum microstephanum	ZINGIBERACEAE
75. cayratia pedata	VITACEAE
76. Hedyotis swersioides	RUBIACEAE
77. H. eualata	RUBIACEAE
78. H.cyanantha	RUBIACEAE
79. H.buxifolio	RUBIACEAE
80. H.barberi	RUBIACEAE
81.Indostristicha tirunelveliana	PODOSTERNACEAE
82. Eria albiflora	ORCHIDACEAE
83. Bulbophyllum kaitiense	ORCHIDACEAE
84. Actinodaphne lawsonii	LAURECEAE
85. A.bourneae	LAURECEAE
86. Rhynchosia velutina	FAEBACEAE
87. Humboldtia laurifolio	FAEBACEAE
88. Cynornetra travancorica	FAEBACEAE
89. Crotolaria scabra	FAEBACEAE
90. C.globosa	FAEBACEAE
91. C.fysonii	FAEBACEAE
92. C.digitata	FAEBACEAE
93. Carex christii	CYBERACEAE
94. Nothopegia aureo	ANACARDIACEAE
95. Aegle marmelos(L.)Correa	RUTACEAE
96. Acacia mearnsii wilde	MIMOSACEAE
97. Adenia hondaia(gaertner) wilde	PASSIFLORACEAE
98. Aleurites moluccana (L.) willd.	EUPHORBIACEAE
99. Asparagus racemosus willd.	LILIACEAE
100. Aloe vera (L.) Burm.f	LILIACEAE
101. Azadirachta indica A. Juss	MELIACEAE
102. Bacopa monnieri (L.) pennell	SCROPHIULARIACEAE
103. Bridelia retusa (L.) spreng	EUPHORBIACEAE
104. Cassia alatie L.	CAESALPINIACEAE
105. Calotropis gigantea (L.) R.Br.	ASCLEPIADACEAE
106. Caralluma adscendens (Roxb.) Haw.Grav.&mayurnathan Vax.attenutata (wight)	ASCLEPIADCEAE
107. Calamus travaneoricus beddome ex Beccari & Hook.b	ARECEACEAE
108. Clitoria ternatea L.	FABACEAE
109. Cardiospermum halicacabum L.	SAPINDACEAE
110. Cinna momum macrocarpum Hook.f.	LAURACEAE
111. Cordia gharof (forsst.) Ehrenb.ex.Asch.	BORAGINACEAE
112. Dalbergia latifolia Roxb.	FABACEAE
113. Dalbergia paniculata Roxb.	FABACEAE
114. Dalbergia sissoides graham ex wight & Arn.	FABACEAE
115. Erythro xylum monogynum Roxb.	ERTHROXYLACEAE
116. Ficus drupacea Thunb. Var.pubeseens (Roth) corner	MORACEAE
117. Ficus tsjahela Burm.f.	MORACEAE
118. Grewia tiliaefolia vahl	TILIACEAE
119. Gymnema sylvestra (Retz.) R.Br.ex schultes	ASCLEPIADACEAE
120. Hibiscus tiliaceus L.	MALVACEAE
121. Jatropha maheshwarii subram.&Nayer	EUPHORBIACEAE
122. Haldina cordifolia (Roxb.) Ridsdale	RUBIACEAE
123. Oxoxylum indicum (L.) Benth.exkury	BIGNONIACEAE
124. Millingtonia hortensis L.f.	BIGNONIACEAE
125. Moringa pterigosperma gaertn.	MORINGACEAE
126. Pedalium murex L.	PEDALIACEAE
127. Ocimum basilicum L.vax.purpureseens Benth.	LAMIACEAE
128. Ocimum tenuiflorum L. (O.sanctum L.)	LAMIACEAE
129. Phyllanthus emblica L.	EUPHORBICEAE
130. Phyllanthus amarus schum. &Thonn.	EUPHORBIACEAE
131. Premna tomentosa willd.	VERBENACEAE
132. Piper longum L.	PIPERACEAE
133. Pterocarpus santalinus L.f.	FABACEAE
134. Radermachera xylocarpa (Roxb.)K.schum.	BIGNONIACEAE
135. Sapindus emarginatus Vahl	SAPINDACEAE
136. Sauropus androgynus (L.) merr.	EUPHORBIACEAE
137. Solanum trilobatum L.	SOLANACEAE
138. Solanum surattense Burm.f.	SOLANACEAE
139. Strychnos nux-vomica L.	LOGANIACEAE
140. Strychnos potatorum L.f.	LOGANIACEAE
141. Stapelia grandiflora masson	ASCLEPIADACEAE
142. Terminalia bellerica (gaertner) Roxb.	COMBRETACEAE
143. Terminalia paniculata Roth	COMBRETACEAE
144. Thottea siliquosa (Lam.) Ding Hou	ARISTOLOCHIACEAE
145. Tribulus terrestris L.	ZYGOPHYLLACEAE
146. Vanila	ORCHIDACEAE
147. Vitex negundo L.	VERBENDACEAE
148. Vitiveria zizanioides (L.) Nash	POACEAE
149. Xylia xylocarpa Taub.	MIMOSACEAE
150. Toona ciliata m.Roem.var.ciliata	MELIACEAE

RESEARCH

Research activities are pursued in the following areas:

Medicinal Plants
Micro propagation & Screening for antimicrobial activity
Environmental Monitoring (Treatment Technology)
Dye Industry Enzymology
Microbial Enzymes with industrial application Oncology
Prostrate cancer
Ovarian Cancer

Publications

V.K. Gopalakrishnan

1. Vishwanatha JK, Salazar E, Gopalakrishnan V.K (2004) Absence of annexin I expression in B-cell non-Hodgkin's lymphomas and cell lines. BMC Cancer Mar 08; 4(1): 8.
2. C. A. Rothermund, V. K. Gopalakrishnan, and J. K Vishwanatha (2004) Androgen Signaling and Post- transcriptional Down-regulation of Bcl-2 in Androgen-unresponsive Prostate Cancer (Prostate Cancer Prostatic Dis. 7(2): 158-64.
3. V.K. Gopalakrishnan and J.K. Vishwanatha (2004) Nicotine inhibits Nitric oxide-induced Apoptosis in Oral epithelial cells (In review Carcinogenesis-2004-00466)
4. C.A. Rothermund, V.K. Gopalakrishnan, J.D. Eudy and J.K. Vishwanatha (2004)) Casodex treatment induces hypoxia-related gene expression in the LNCaP prostate cancer progression model (In review Cancer Res.)
5. A.G. Banerjee, Jie Liu, Yawei Tuan, V.K. Gopalakrishnan, S.L. Johansson, A.K. Dinda, N.P. Gupta, Lindsey Trevino and J.K. Vishwanatha (2003) Expression of biomarkers modulating prostate cancer angiogenesis: Differential expression of annexin II in prostate carcinomas from India and USA (Molecular Cancer, 2:34)
6. V. K. Gopalakrishnan, A. G. Banerjee, and J. K. Vishwanatha (2003) Effect of Fhit replacement on growth, cell cycle and apoptosis in pancreatic cancer cells. Pancreatology, 3:293-302
7. Abhijit G. Banerjee, V. K. Gopalakrishnan, I. Bhattacharyya and J.K.Vishwanatha (2002) Deregulated cyclooxygenase-2 expression in oral premalignant tissues. Mol. Cancer Ther., 1:1265-1271.
8. E. Babu, V. K. Gopalakrishnan, I. Navis Paul Sriganth, R. Gopalakrishnan and D. Sakthisekaran (1995). Cisplatin induced nephrotoxicity and modulating effect of glutathione ester. Molecular and Cellular Biochemistry, 144: 7-11.
9. V.K.Gopalakrishnan, I.Navis Paul Sriganth, N.Chandra Mohan and D.Sakthisekaran (1994). Modulatory effect of sodium selenite in fibrosarcoma bearing rats treated with cisplatin with reference to membrane bound ATPases. Biomedicine, 14: 23-28.
10. V.K.Gopalakrishnan, I. Navis Paul Sriganth and D.Sakthisekaran (1993). Effect of cis- diammine dichloroplatinum - II (Cisplatin) and sodium selenite on renal antioxidant enzymes of experimental fibrosarcoma rats. Medical Science Research, 21:647-649.
11. V.K.Gopalakrishnan and D.Sakthisekaran (1991). Effect of patulin on albumin fractions of plasma protein studies in rats. Biochemistry International, 25: 461-475.

Mrs.C.Uma

1. Production and characterization of glucoamylase from Aspergillus sydowii. Advances in Plant Sciences vol. 19, 2006.

2. Antimicrobial activity of traditional medicinal plants from Southern Western Ghats. Asian Journal of Microbiology, Biotechnology and Environmental Sciences. Vol 4, 2005.

3. Adsorption kinetics of direct violet-47 (dye) on activated carbon obtained from low-cost agricultural waste. Asian Journal of Microbiology, Biotechnology and Environmental Sciences. Vol 4, 2005.

Mrs.K.Devaki

1. ”Production of Cellulase from Aspergillus niger and
Bacillus subtilis using Pineapple peel as subsrate by solid state fermentation” in Asian Journal of Microbiology, Biotechnology and Environmental Sciences, Vol 3, 2006

2. ”Comparative study ot Etanol production by the free cells of zymomonas mobilis and Saccharomyces cerevisiae from sugarcane” in Asian Journal of Microbiology, Biotechnology and Environmental Sciences, Vol 4, 2006

M.Sunitha

1.Production of Cellulase from Aspergillus Niger and Bacillus subtilis using Pineapple peel as substrate by solid state fermentation in Asian Journal of Microbiology, Biotechnology and Environmental Sciences, Vol 8,No.3, 2006

2.Comparative study of Ethanol Production by the free cells of Zymomonas mobilis and Saccharomyces cerevisiae fromsugarcane in Asian Journal of Microbiology,
Biotechnology and Environmental Sciences, Vol 8,No.4 2006.

M.Kalyani

1. Adsorption kinetics of direct violet-47 (dye) on activated carbon obtained from low-cost agricultural waste in Asian Journal of Microbiology, Biotechnology and Environmental Sciences, Vol 4, 2005.

LINKAGES

1. Thyrocare, Mumbai
2. Centre for Medicinal Plants Research, Kottakkal.
3. KMC Hospital, Mangalore.
4. Bacto Agro Culture Care Pvt Ltd, Kolapally, Nilgiris.
5. National Centre For Biological Sciences, Bangalore..
6. Salim Ali Centre For Ornithology and Natural History, Anaikatty, Coimbatore.
7. BMERF, Salem.
8. Regional Research Laboratory, Trivandrum.
9. Indian Institute Of Spices Research, Calicut.
10. Central Tuber Crops Research Institute, Thiruvanathapuram.
11. Amala Institute Of Medical Sciences, Thrissur.
12. Best Biotek Research Labs, Bangalore.
13. Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram.
14. The Karnataka Cancer Therapy & Research Institute, Hubli.
15. JIVAS, Bangalore.
16. Central Institute Of Fisheries Technology, Cochin.
17. Bhabha Atomic Research Centre, Mumbai.
18. Senthil Papain and Food Products, Coimbatore.
19. Indian Institute of Sciences, Bangalore.
20. Cashew Export and Promotion Council of India, Kollam.